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Citing the available literature, Deepankar et al

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The supernatant was discarded, and the pellet resuspended in cold PBS + 1% Triton X-100 plus protease and phosphatase inhibitor cocktails, transferred to an Eppendorf tube and allowed to rotate for 30 minutes at 4C

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Ravanfar R, Sheng Y, Gray HB, Winkler JR